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Year : 2023  |  Volume : 8  |  Issue : 2  |  Page : 134-142

Seasonal assessment of asiaticoside content in Centella asiatica (L.) Urb. by HPLC and pharmacognostical studies to establish its best procurement time

1 Department of Chemistry, CCRAS-Captain Srinivas Murthy Central Ayurveda Research Institute, Chennai, India
2 Department of Pharmacognosy, CCRAS-Central Ayurveda Research Institute, Kolkata, India
3 Department of Chemistry, CCRAS-Captain Srinivasa Murthy Central Ayurveda Research Institute, Chennai, India
4 Department of Pharmacology, CCRAS-Captain Srinivasa Murthy Central Ayurveda Research Institute, Chennai, India
5 Central Council of Research in Ayurvedic Sciences, New Delhi, India

Correspondence Address:
Dr. Ch Venkata Narasimhaji
Department of Chemistry, CCRAS-Captain Srinivasa Murthy Central Ayurveda Research Institute (CSMCARI), M/o AYUSH, Govt. of India, A. Anna Hospital Campus, Arumbakkam, Chennai-106
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jdras.jdras_84_21

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BACKGROUND: This study was designed to validate the collection time for the whole plant of Mandookparni [Centella asiatica (L.) Urb.] by assessing the variation in marker compound asiaticoside, a bioactive secondary metabolite with quantitative high performance liquid chromatography in all available seasons along with the observation of morphological and microscopic studies. METHODS: Centella asiatica (L.) Urb. the whole plant was collected from the same habitat in all six seasons described in Ayurveda that is Shishir ritu (Jan–Feb), Vasant ritu (Mar-Apr), Grishm ritu (May-Jun), Varsha ritu (July-Aug), Sharad ritu (Sep-Oct), and Hemant ritu (Nov–Dec). The source of collected plant material was authenticated at Central Ayurveda Research Institute (CARI), Jhansi. The voucher specimen was deposited in the CARI herbarium (Accession no. 23602) for future reference. Botanical identification and comparative macro and microscopic studies for samples collected in each season were carried out. The extraction in various solvents such as alcohol, hydro alcohol, and methanol through soxhlet was carried out to evaluate the quantitative analysis of extractive values and asiaticoside in all six seasons through HPLC at Captain Srinivasa Murthy Central Ayurveda Research Institute (CSMCARI), Chennai. RESULTS: The whole plant is used for medicinal purpose. It was observed that the flowering and fruiting occur at end of the rainy (Varsha ritu) season and persists up to the winter season (Shishir ritu). Plant material is procured from the same habitat in all seasons from Central Ayurveda Research Institute (CARI) garden, Jhansi. The raw botanical material showed the similar characters in powder microscopy in Shishir ritu, Vasant ritu, Sharad ritu, and Hemant ritu whereas the powder study of Grishma ritu and Varsha ritu was observed without the fruiting parts that is pericarp and endosperm. And other four seasons are same as Shishir ritu. Selected marker compound asiaticoside was quantified in all available seasons by HPLC. The extractive value was found maximum of 2.2332 g (44.66% w/w) in a methanol extract of Grishma ritu sample and HPLC estimation evident for the large quantity of asiaticoside is more upto 0.4596 (% w/w) in Grishma ritu sample. CONCLUSION: The highest concentration of asiaticoside was found in the sample collected during Grishma ritu (May and June). Hence, it can be concluded that the best procurement time for Mandukaparni whole plant is Grishma ritu (May and June).

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