• Users Online: 432
  • Print this page
  • Email this page
Year : 2023  |  Volume : 8  |  Issue : 2  |  Page : 124-133

Preconditioning with Partharishta reduces the oxidative stress associated with myocardial ischemic reperfusion injury in H9c2 cells

Department of Rasashastra and Bhaishajya Kalpana, Government Ayurveda College, Trivandrum, Kerala, India

Correspondence Address:
Dr. M Gowri
Department of Rasashastra and Bhaishajya Kalpana, Government Ayurveda College, Trivandrum, Kerala
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jdras.jdras_25_21

Rights and Permissions

BACKGROUND: Myocardial Ischemia Reperfusion (I/R) injury- a paradoxical challenge associated with contemporary methods of successful resuscitation triggers uncontrolled apoptosis induced by the concomitant oxidative stress. Adaptive mechanisms incorporating the upregulation of cytoprotective genes like Nrf2 protects cells from apoptotic changes and dysfunction under stress conditions. Partharishta an Ayurvedic formulation with all its individual ingredients being potent antioxidants, is thought to bring a positive result in reducing oxidative stress in cardiac tissues. Therefore the study aims to evaluate the antioxidant activity of the formulation Partharishta via the expression of Nrf2 gene in ischemia induced H9c2 rat cardiomyoblast cells when preconditioned with Partharishta. METHODS: Partharishta was prepared in accordance with the Ayurvedic Formulary of India (AFI). To evaluate the extent of cell viability the Partharishta pretreated H9C2 cell lines were subjected to MTT Assay. As confirmatory assays for antioxidant activity, an invitro ROS measurement assay and Reverse transcriptase PCR Analysis to confirm the expression of Nrf2 gene against oxidative stress was conducted. RESULTS: On MTT Assay the cells pretreated with 25µg/ml of dessicated Partharishta provided an average cell viability of 85.12%. The confirmatory Assays conducted in the cells at this concenteration showed that the ROS production is relatively low and the Nrf2 gene against oxidative stress was expressed in these cells. CONCLUSION: These findings of the present in-vitro study reveal that the formulation Partharishta possess significant antioxidant as well as the free radical scavenging activity and therefore may be considered as one of the good sources of natural cardioprotective formulation.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded118    
    Comments [Add]    

Recommend this journal