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VOLUME 5 , ISSUE 4 ( October-December, 2020 ) > List of Articles
Raghavendra Naik, CR Harisha, Rabinarayan Acharya
Keywords : Dendrobium macraei Lindl., Jīvantī, Pharmacognosy, Random amplified polymorphic DNA analysis
Citation Information : Naik R, Harisha C, Acharya R. Pharmacognostical Study and Random Amplified Polymorphic DNA Analysis of Dendrobium macraei Lindl.—A Source Plant of Jīvantī. J Drug Res Ayurvedic Sci 2020; 5 (4):197-202.
License: CC BY-NC 4.0
Published Online: 22-01-2021
Copyright Statement: Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.
Aim: Dendrobium macraei Lindl. is considered as one of the source drugs of jīvantī in some parts of India. The plant is used for different diseases like skin allergy, eczema, snakebite, and as an aphrodisiac. The present study was carried out to establish the pharmacognostical profile and molecular characterization of leaves of D. macraei Lindl. Materials and methods: Fresh leaves of D. macraei Lindl. were studied for pharmacognostical characters following standard guidelines and molecular characterization was established by random amplified polymorphic DNA (RAPD) markers. Results: A microscopic study of leaves shows the presence of a single layer of undifferentiated upper and lower epidermis covered with a thick, rigid cuticle. Mesophyll tissue is filled with undifferentiated isodiametric parenchyma, mucilage cavities, and raphide idioblast. The large vascular bundle is located centrally in the midrib portion and smaller vascular bundles pass through the main nerves. Powder microscopy showed diagnostic characters like epidermal cells, stomata, acicular crystals, prismatic crystals, rhomboidal crystals, and starch grain with hilum. Histochemical tests confirm the presence of lignified cells, starch, calcium oxalate crystals, tannin, and oil globules. In RAPD analysis, all the 10 primers showed good amplification. Conclusion: These microscopic observations and the unique bright and light bands obtained in polymerase chain reaction (PCR) amplification could serve as a measure for authentication and standardization of the plant.