Journal of Drug Research in Ayurvedic Sciences

Register      Login

VOLUME 4 , ISSUE 4 ( October-December, 2019 ) > List of Articles


Vasa (Justicia adhatoda L.) Leaves Best Procurement Time with Special Reference to Assay of Vasicine through HPLC and its Comparative Pharmacognosy

Narayana Srikanth

Keywords : High-performance liquid chromatography, Phytochemical studies,Best procurement time

Citation Information : Srikanth N. Vasa (Justicia adhatoda L.) Leaves Best Procurement Time with Special Reference to Assay of Vasicine through HPLC and its Comparative Pharmacognosy. J Drug Res Ayurvedic Sci 2019; 4 (4):168-174.

DOI: 10.5005/jdras-10059-0082

License: CC BY-NC 4.0

Published Online: 00-12-2019

Copyright Statement:  Copyright © 2019; Jaypee Brothers Medical Publishers (P) Ltd.


Aim: Vasa (Justicia adhatoda L.) is a well-known plant for its medicinal properties, e.g., ailments of asthma, bronchitis, cough, dyspnea, external bleeding, etc. Different preparations of vasa are described in the Ayurvedic texts to pacify these disorders. In Ayurveda, the season of collection of its useful parts, i.e., leaves are not mentioned anywhere in the ancient texts. Since some research claim higher concentration of its active metabolite in winter and rainy season, therefore, it has been planned to evaluate the suitable time of collection of plant parts. Materials and methods: The leaves of vasa (Justicia adhatoda L.) were collected from the same habitat in all six seasons described in Ayurveda, i.e., shishir (Jan to Feb), vasant (Mar to Apr), grishm (May to June), varsha (Jul to Aug), sharad (Sept to Oct), and hemant (Nov to Dec). The source of the collected plant materials was authenticated at Regional Ayurveda Research Institute, RARI Jhansi, India, through the herbarium (accession no. 23595). Identification, comparative macroscopic, and microscopic along with powder microscopy of the leaves of the plant in each season were carried out besides extraction of various solvents such as alcohol, hydroalcohol, and methanol though Soxhlet and its comparative quantitative analysis for the extracted material in all six seasons through high-performance liquid chromatography (HPLC) for the different seasons. Results: Pharmacognostical characteristics remain the same in all seasons, while the selected marker compound vasicine varied in quantity in each season. Extractive value was found maximum, i.e., 1.3456 g in vasant ritu sample and also evident in the quantitative HPLC estimation for the abundant vasicine in vasant ritu sample, i.e., 0.7330 to 1.0744 which is the clear indication for the best time to collect leaves in vasant ritu. Conclusion: In vasant ritu, vasa (Justicia adhatoda L.) is in flowering stage, and at this stage the abundance of the secondary metabolites and the extractive values clearly indicate to conclude that the suitable procurement time of collection of vasa leaves (Justicia adhatoda L.) is vasant ritu (Mar to Apr).

PDF Share
  1. Sharma PV. Dravyaguna-Vigyana. Vegetable Drugs, vol. II, Varanasi: Chaukambha Bharati Academy; 2001. p. 241–244.
  2. Soni S, Anandjiwala S, Patel G, et al. Validation of different methods of preparation of Adhatoda vasica leaf juice by quantification of total alkaloids and Vasicine. Indian J Pharm Sci 2008;70(1):36–42. DOI: 10.4103/0250-474X.40329.
  3. Charakasamhita by Agnivesha revised by Charaka and Dridhbala with Ayurveddipika Commentary of Chakrapanidatta, ed. by Yadavji Trikamji Acharya, Varanasi, Chaukhambha Sanskrit Sansthan, 1984, Chikitsa 4/63.
  4. Suśruta Samhitā of Maharsi Suśruta with Bhanumati commentary by Chakrapanikdatta, ed. By Acharya Y.T. and Sharma N.K. Pub. By Pt. Sharma SS, Registrar, Agra University, Secretary Swami Laxmi Ram Trust: 1939, Uttar tantra 41/43.
  5. Shah BG, Nighantu Adarsha; Uttarardh Vasadi verga, Chaukhambha Prakashana, Varanasi, 1968. p. 204.
  6. Aśtāngahrdayam of Vāgbhata, Vidyotini Commentary by Kaviraj Atrideva Gupta, ed. Yadunandana Upadhyaya, Chaukhambha Sanskrit Sansthan, Varanasi, India, 8th ed., 1982. A.H.Su 6/78,).
  7. Mudgal V, Khanna KK, Hajra PK. Flora of Madhya Pradesh, vol. 2, Calcatta: Botanical Survey of India Calcatta; 1997. p. 284.
  8. Sandeep P, Ritu O, Gurpreet K, et al. Estimation of seasonal Variation of two major Pyrrolo (2,1-b) Quinazoline alkaloids of Adhatoda vasica by HPLC. Nat Prod J 2013;3(1):30–34.
  9. Evans WC. Trease and Evans Pharrmacognosy. 15 ed., London: WB Saunders Ltd; 2002.
  10. Khandelwal KR. Practical Pharmacognosy. 18th ed., Pune: Nirali Prakashan; 2007.
  11. Khasim SM. Botanical Microtechnique: Principles & Practice. New Delhi. Capital Publishing Company; 2002.
  12. Raja S, Unnikrishnan. KP, George S, et al. Variation in Vasicine content and Pharmacognostic characters of Morphotypes of Adhatoda zeylanica medic. J Plant Sci 2008;3(1):61–68. DOI: 10.3923/jps.2008.61.68.
  13. Bhide MB, Naik PY, Ghooi RB. Comparative studies on the pharmacological studies on the pharmacological evaluation of vasicine and vasicinone. Bull Huff Inst 1976;4:45–50.
  14. Srivastava SK, et al. HPLC determination of vasicine and vasicinone in Adhatoda Vasica with photo diode array Detection. J Liq Chrom & Rel Technol 2001;24(2):153–159. DOI: 10.1081/JLC-100001478.
  15. Anonymous, Quality standard of Medicinal Plants. vol. 3, New Dehli: Medicinal Plants unit ICMR; 2005(1): p. 1–8.
  16. Gupta A, Joshi A, Joshi VK. Pharmacognostical study of Justicia adhatoda linn. leaf. international. J Herbal Med 2014;1(6):01–04.
  17. Kanthale PR, Panchal VH. Pharmacognostic study of Adhatoda vasica Nees. bioscience. Discovery 2015;6(1):49–53.
  18. Anonymous, Database on medicinal plants used in Ayurveda, New Delhi, CCRAS Publication vol. 1, 498.
  19. Chatterjee A, Pakrashi SC. The Treatise of Indian Medicinal Plants, vol. 5, New Delhi: NISCAIR; 2003. p. 48.
  20. Satyavati GV, Gupta AK, Tandon N. Medicinal plants of India, vol. II, New Delhi: Indian Council of Medicinal Research; 1987.
  21. Anonymous; Reviews on Indian medicinal plants vol. 1, New Delhi, Medicinal Plants unit, Indian council of Medical Research, 261.
  22. Anonymous; Indian pharmacopoeia 2014, vol. III, New Delhi, Indian Pharmacopoeia Commission, Ministry of Health and family Welfare. Govt. of India. p. 3267–3268.
  23. Anonymous, Ayurvedic pharmacopoeia of India, Part–I: vol. 8, Govt. of India, Mnistry of Health and family welfare, Department of AYUSH, 2011, 164.
PDF Share