Journal of Drug Research in Ayurvedic Sciences

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VOLUME 4 , ISSUE 3 ( July-September, 2019 ) > List of Articles

RESEARCH ARTICLE

Multiplication of Bharangi—Clerodendrum serratum (L.) Moon: An Ayurvedic Important Plant

Arun M Gurav, Archana G Mhase, Rekha R Nirawane, Rasika Kolhe, Penchala P Goli, Anupam K Mangal

Keywords : Growth regulators, In vitro propagation, Micropropagation, Node,Conservation

Citation Information : Gurav AM, Mhase AG, Nirawane RR, Kolhe R, Goli PP, Mangal AK. Multiplication of Bharangi—Clerodendrum serratum (L.) Moon: An Ayurvedic Important Plant. J Drug Res Ayurvedic Sci 2019; 4 (3):126-131.

DOI: 10.5005/jdras-10059-0078

License: CC BY-NC 4.0

Published Online: 00-09-2019

Copyright Statement:  Copyright © 2019; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: To develop effective in vitro multiplication protocol for rare and endangered medicinal plants of Clerodendrum serratum (L.) Moon. Materials and methods: Trials were carried out using nodal segments as explants, which were inoculated on Murashige and Skoog medium (MS) plain medium and different concentrations of phytohormones viz., benzylaminopurine (BAP), kinetin (Kn), indol-3-butyric acid (IBA), indol-3-acetic acid (IAA), and naphthaleneacetic acid (NAA), and incubated for 8 hours of photoperiod using cool- white fluorescent tubes with a light intensity of 3,000 lux at 22°C + 2°C. Results: The maximum (7.0 ± 0.045) numbers of shoots were developed from the nodal segment inoculated on MS fortified with BAP (4 mg/L) ± 0.1 % polyvinylpyrrolidone (PVP) with shoots of 1.72 ± 0.018 cm height. Auxin BAP at 4 mg/L proved to be effective for bud proliferation and production of multiple shoots. The best and early root induction was achieved on MS + IBA (1–4 mg/L). Conclusion: The developed in vitro protocol would be beneficial for the fast multiplication of bharangi plants and help to minimize the burden on supply and demand, so that the huge demand for the drug is fulfilled and also helpful to protect the plant.


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  1. Cooke T. The flora of the Presidency of Bombay. 2nd reprint, vol. I, II and III, Calcutta: Botanical Survey of India; 1967. p. 54.
  2. Singh NP, Karthikeyan S, Lakshminarasimhan P, et al. Flora of Maharashtra State-Dicotyledons, vol. 2, Calcatta: Botanical Survey of India; 2001. pp. 162–163.
  3. Haritakyadi Varga/182. Bhavapraaksh Nighantu. Chaukhambha Bharati Academy-Varanasi. p. 101.
  4. Sharma PC, Yelne MB, Dennis TJ. Database on medicinal plants used in ayurveda. vol. 5, New Delhi: Central Council for Research in Ayurveda & Siddha; 2002. pp. 171–186.
  5. Poornima BS, Hegde PL, Pradeep HA. Pharmacological review on Clerodendrum serratum Linn. Moon. Journal of Pharmacognosy and Phytochemistry 2015;3(5):126–130.
  6. Kirtikar, Basu. Indian Medicinal Plant, vol. III, Deharadun: International Book Distributors; 1999. p. 1948.
  7. Frlht.org. (2019). Foundation for Revitalization of Local Health Traditions. [online] Available at: http://www.frlht.org/ [accessed 18 Aug. 2019].
  8. Rao RR, Sharma BD. A Manual for Herbarium collections. Calcutta: Botanical Survey of India; 1990. pp. 5–20.
  9. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 1962;15(3):473–497. DOI: 10.1111/j.1399-3054.1962.tb08052.x.
  10. Vidya SM, Krishna V, Manjunatha BK, et al. Micropropagation of Clerodendrum serratum L. through direct and indirect organogenesis. Plant Tissue Culture and Biotechnology 2012;22(2):179–185. DOI: 10.3329/ptcb.v22i2.14208.
  11. Vijay R, Shukla J, Saxena R. In vitro effect of various plant growth promoters on clerodendrum serratum. Int J Pure App Biosci 2016;4(6):186–192. DOI: 10.18782/2320-7051.2409.
  12. Upadhyay S, Koche V. Comparison of different medium and establishment of an efficient micropropagation technique of Clerodendrum serratum L. an endangered medicinal plant. Journal of Environmental Science, Toxicology and Food Technology 2015;1(1):27–35.
  13. Sharma M, Rai SK, Purshottam DK, et al. In vitro clonal propagation of Clerodendrum serratum (linn.) moon (barangi): a rare and threatened medicinal plant. Acta Physiol Plant 2009;31(2):379–383. DOI: 10.1007/s11738-008-0245-4.
  14. Sahoo Y, Chand PK. Micropropagation of vitex negundo L., a woody aromatic medicinal shrub, through high-frequency axillary shoot proliferation. Plant Cell Rep 1998;18(3-4):301–307. DOI: 10.1007/s002990050576.
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