Journal of Drug Research in Ayurvedic Sciences

Register      Login

VOLUME 4 , ISSUE 2 ( April–June, 2019 ) > List of Articles

RESEARCH ARTICLE

Development of a Novel Polyherbal Formulation for Augmenting Milk Production in Healthy Dairy Cows

Manajit Bora, Bhavana Srivastava, Sudesh N Gaidhani, Himanshu Sharma, Manish K Gautam, Rajesh K Tiwari, Manish M Wanjari, Jayram Hazra

Keywords : Galactagogue, High performance thin layer chromatography, Polyherbal formulation, Standardization,Dairy

Citation Information : Bora M, Srivastava B, Gaidhani SN, Sharma H, Gautam MK, Tiwari RK, Wanjari MM, Hazra J. Development of a Novel Polyherbal Formulation for Augmenting Milk Production in Healthy Dairy Cows. J Drug Res Ayurvedic Sci 2019; 4 (2):84-94.

DOI: 10.5005/jdras-10059-0064

License: CC BY-NC 4.0

Published Online: 00-06-2019

Copyright Statement:  Copyright © 2019; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: The study was designed to develop and standardize a novel polyherbal formulation (PHF) for augmenting milk production in healthy dairy cattle. Materials and methods: Five raw plant drugs, viz., tubers of Asparagus racemosus Willd. (Shatavari), whole plant of Eclipta alba (L.) Hassk. (Bhringraj), seeds of Trigonella foenum-graecum L. (Methika), fruits of Foeniculum vulgare Mill. (Mishreya.), and Anethum sowa Roxb. ex Fleming (Shatapushpa) were used to prepare hydroalcoholic extracts using the Soxhlet method. Three in-house batches of PHF were prepared and standardized as per Ayurvedic Pharmacopoeia of India (API) methods. Pharmacognostic authentication and chemical identification were done by macroscopic and microscopic studies, phytochemical screening, physicochemical analysis, and high performance thin layer chromatography (HPTLC) fingerprinting. The safety studies of galactagogue preparation were performed through heavy metals, microbial contamination, aflatoxins, and pesticide residue analysis. Results: Organoleptic studies revealed that all the batches appeared as semisolid in nature, blackish-brown in color, with a pleasant odor and slight bitter taste. Phytochemical screening confirmed the presence of similar secondary metabolites in the different batches of both raw drugs and PHF. Physicochemical analysis and HPTLC fingerprints at different illuminations showed that all three batches were uniformly composed and complied the pharmacopeial limits. Results of safety parameters advocated that all the three batches were safe and complied as per the WHO and API guidelines. Conclusion: The present work first claims the standardization of this unique, cost-effective, nonhormonal, Ayurvedic galactagogue in-house preparation, i.e., PHF for augmenting milk yield in dairy herd. It proves that all the three batches have similar characteristics and uniformly composed. It serves as a reference for identification and distinguishing the galactagogue herbs.


PDF Share
  1. Felipe PT, Juliana VBJ, Zulma TRC. Pharmacological overview of galactogogues. Vet Med Int 2014;2014:602894. Available from http://dx.doi.org/10.1155/2014/602894.
  2. Melnyk M. Recombinant bovine somatotropin: challenging Canada’s science-based regulatory system and the emergence of post-normal science [Doctoral, thesis], Department of Sociology. Saskatoon, Canada: University of Saskatchewan; 2005.
  3. Westfall RE. Galactagogue herbs: qualitative study and review. Can J Mid Res Prac 2003;2(2):22–27.
  4. Latvietis J, Drikis V, Auzins AT, et al. Some types of grass silage used in feeding cows. Tartu, Estonia: Proceedings of the Animal Nutritions Conference; 2002; pp. 7–15.
  5. Alamer M. Effect of feeding fennel straw (Foeniculum vulgare mill) on performance of lactating goats. J App An Res 2009;36(1):61–64. DOI: 10.1080/09712119.2009.9707032.
  6. Kholif M, Abd El-Gawad MAM. Medical plant seeds supplementation of lactating goat’s diets and its effects on milk and cheese quantity and quality. Egypt J Dairy Sci 2001;29:139–150.
  7. Tomar KS, Singh VP, Yadav RS. Effect of feeding maithy (Trigonella foenum-graecum) and chandrasoor (Lepidiumsativum L.) seeds on milk and blood constituents of Murrah buffaloes. Ind J An Sci 1996;66(11):1192–1193.
  8. Ryd’en G, Oholm S. Half-life of oxytocin in blood of pregnant and non pregnant women. Acta Endocrin 1969;61(3):425–431. DOI: 10.1530/acta.0.0610425.
  9. Westfall RE. Galactagogue herbs: a qualitative study and review. Can J Mid Res Prac 2003;2(2):22–27.
  10. Abascal K, Yarnell E. Botanical galactagogues. Alt Comp Ther 2008;14(6):288–294. DOI: 10.1089/act.2008.14602.
  11. Mortel M, Mehta SD. Systematic review of the efficacy of herbal galactogogues. J Hum Lact 2013;29(2):154–162. DOI: 10.1177/0890334413477243.
  12. Patel MD, Tyagi KK, Sorathiya LM, et al. Effect of polyherbal galactogogue supplementation on milk yield and quality as well as general health of Surti buffaloes of south Gujarat. Vet World 2013;6(4):214–218. DOI: 10.5455/vetworld.2013.214-218.
  13. Singhal SP. Study on the effect of feeding Payapro on milk yield in lactating cows. Dairy Guide 1995;1:45–47.
  14. Qureshi MI. Efficiency of Lectovet as a galactagogue in dairy cattle. Indian Vet Med J 1999;23:134–136.
  15. Bhatt N, Singh M, Ali A. Effect of feeding herbal preparations on milk yield and rumen parameters in lactating crossbred cows. International J Agri Biol 2009;11(6):721–726.
  16. Agrawala IP, Achar MV, Boradkar RV, et al. Galactagogue action of Cuminum cyminum and Nigella sativa. Ind J Med Res 1968;56(6):841–844.
  17. Behera PC, Tripathy DP, Parija SC. Shatavari: Potentials for galactogogue in dairy cows. Indian J Trad Know 2013;12(1):9–17.
  18. Mishra UK, Kanesh JS, Mandal AK, et al. Potentials of herbal galactogogues in milk production in ruminants. The Ind Cow 2006; 44–52.
  19. Mohanty I, Senapati MR, Jena D, et al. Ethnoveterinary importance of herbal galactogogues - a review. Vet World 2014; 325–330. DOI: 10.14202/vetworld.2014.325-330.
  20. Anonymous. Medicinal Plants of India. Indian Council of Medical Research. New Delhi: Govt. of India; 1985; p. 285.
  21. Gbadamosi IT, Okolosi O. Botanical galactogogues: Nutritional values and therapeutic potentials. J Appl Biosci 2013; 4460–4469. DOI: 10.4314/jab.v61i0.85594.
  22. Tiran D. The use of fenugreek for breast feeding women. Comp Ther Nurs Mid 2003;9:155–156. DOI: 10.1016/S1353-6117(03)00044-1.
  23. Sreeja S, Anju VS. In vitro estrogenic activities of fenugreek Trigonella foenum graecum seeds. Ind J Med Res 2010;131(1):814–819.
  24. El-Alamy A, Khattab HM, El-Nor SA, et al. Milk production response to supplementing rations with some medical herbs of lactating buffaloes. Proceedings of the 8th Egyptian Conference for Dairy Science and Technology. Cairo, Egypt 2001; pp. 675–686.
  25. Alamer MA, Basiouni G. Feeding effects of fenugreek seeds (Trigonella foenum-graecum L) on lactation performance, some plasma constituents and growth hormone level in goats. Pak J Biol Sci 2005;25(11):28–46.
  26. Albert-Puleo M. Fennel and anise as estrogenic agents. J Ethnopharm 1980;2(4):337–344. DOI: 10.1016/S0378-8741(80)81015-4.
  27. Sharma K, Bhatnagar M. Asparagus racemosus (shatavari): a versatile female tonic. Int J Pharm Biol Arch 2011;2(3):855–863.
  28. Gupta M, Shaw B. A double-blind randomized clinical trial for evaluation of galactogogue activity of asparagus racemosus Willd. Iran J Pharm Res 2011;10(1):167–172.
  29. Pandey SK, Sahay A, Pandey RS, et al. Effect of Asparagus racemosus rhizome (shatavari) on mammary gland and genital organs of pregnant rat. Phyto Res 2005;19(8):721–724. DOI: 10.1002/ptr.1590.
  30. Patel AB, Kanitkar UK. Asparagus racemosus Willd—form bordi, as a galactogogue, in buffaloes. Ind Vet J 1969;46(8):718–721.
  31. Saxena G, Singh M, Bhatnagar M. Phytoestrogens of Asparagus racemosus wild. J Herb Med Toxico 2010;4(1):15–20.
  32. Westfall RE. Galactagogue herbs: a qualitative study and review. Can J Mid Res Pract 2003;2(2):22–27.
  33. Bharti SN, Sharma NK, Gupta AK, et al. Pharmacological actions and potential uses of diverse galactogogues in cattle. Int J Cl Pharm Ther 2012;2(1):24–28.
  34. Underwood MA. Human milk for the premature infant. Ped Cl North Amer 2013;60(1):189–207. DOI: 10.1016/j.pcl.2012.09.008.
  35. Zapantis A, Steinberg JG, Schilit L. Use of herbals as galactagogues. J Pharm Pract 2012;25(2):222–231. DOI: 10.1177/0897190011431636.
  36. Baig MI, Bhagwat VG. Study the efficacy of galactin vet bolus on milk yield in dairy cows. Vet World 2009;2(4):140–142.
  37. Gurib-Fakim A. Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Asp Med 2006;27(1):1–93. DOI: 10.1016/j.mam.2005.07.008.
  38. Chan K. Some aspects of toxic contaminants in herbal medicines. Chem 2003;52(9):1361–1371.
  39. Verma S, Singh SP. Current and future status of herbal medicines. Vet World 2008;1(11):347–350. DOI: 10.5455/vetworld.2008.347-350.
  40. WHO. General guidelines for methodologies on research and evaluations of traditional medicine. Geneva: World Health Organization; 2000.
  41. Pushpendra, Kumar KNS, Priyadarshini, et al. Quality standards for hutabhugadi churna (ayurvedic formulary of India). J Trad Comp Med 2016;6(1):78–88.
  42. Kunle OF, Egharevba HO, Ahmadu PO. Standardization of herbal medicines-a review. Int J Biodivers Conserv 2012;4:101–112. DOI: 10.5897/IJBC11.163.
  43. Oruganti M. Organic dairy farming: A new trend in dairy sector. Vet World 2011;4(3):128–130. DOI: 10.5455/vetworld.2011.128-130.
  44. Trease GE, Evans WC. Textbook of pharmacognosy 12th ed., London: Balliese Tindall and Company Publisher; 1983; pp. 343–383.
  45. Ramesh PT, Mitra SK, Suryanarayna T, et al. Evaluation of galactin - a herbal galactogogue, preparation in dairy cows. The Vet 2001;2(4):1–3.
  46. Schaneberg BT, Khan IA. Analysis of products suspected of containing Aristolochia or Asarum species. J Ethnopharm 2004;94(2-3):245–249. DOI: 10.1016/j.jep.2004.06.010.
  47. Perry LM. Medicinal plants of East and Southeast Asia. London: Cambridge (Mass); 1980.
  48. Bruckner C. A survey on herbal galactogogues used in Europe. Med AliHeid: Appr Ethnopharm 1993; 140–145.
  49. Tabasum S, Khare S. Safety of medicinal plants: an important concern. Int J Pharm Bio Sci 2016;7:237–243.
  50. Lachumy SJ, Sasidharan S. Current microscopy contributions to advances in science and technology. Spain: Formatex Research Center, vol. 1 2012; pp. 704–710.
  51. WHO. Quality control methods for herbal materials, 1st ed., Geneva: WHO Press; 1998.
PDF Share