Journal of Drug Research in Ayurvedic Sciences

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VOLUME 3 , ISSUE 4 ( October-December, 2018 ) > List of Articles


Pharmacognostical, DNA Barcoding, and Phytochemical Analysis on Leaves of Leea macrophylla Roxb. ex Hornem

Switu V Jani, Harisha C Rudrappa, Vinay J Shukla

Keywords : Hanshia dabar, Hastikarna palasa, Leaf, Leea macrophylla,Anukta dravya

Citation Information : Jani SV, Rudrappa HC, Shukla VJ. Pharmacognostical, DNA Barcoding, and Phytochemical Analysis on Leaves of Leea macrophylla Roxb. ex Hornem. J Drug Res Ayurvedic Sci 2018; 3 (4):214-227.

DOI: 10.5005/jdras-10059-0054

License: CC BY-NC 4.0

Published Online: 00-12-2018

Copyright Statement:  Copyright © 2018; Jaypee Brothers Medical Publishers (P) Ltd.


Aim: Hanshia dabar or hatkhan is one of the folklore plants of Odisha, which has been identified as Leea macrophylla Roxb. ex Hornem. of the family Vitaceae. Traditionally, its leaves are claimed to be used in the treatment of cancer. Pharmacognostical evaluation of various parts of the plants has not been reported. The present study has been designed to carry out detailed macro- and microscopic, physiochemical, and phytochemical aspects of leaves of L. macrophylla. Materials and methods: The fresh leaves, collected from Gandhamardan hills, Odisha, during the month of September 2016 were examined for its pharmacognostical characters and physicochemical, qualitative analysis, and HPTLC studies were done following the standard protocols recommended by the Ayurvedic Pharmacopoeia of India. Results: Leaves are compound, unipinnate, leaflets two pairs, and one terminal with short petiolule. Transverse section of rachis resembles the anatomy of stem and that of petiolule is somewhat deeply lobed heart shaped, section passing through midrib resembles inverted bell shape with two wings, one anterior and other posterior both ending with tapering ends. Leaf powder is greenish in color and microscopy reveals the presence of paracytic stomata. The plant is identified as L. macrophylla based on sequence homology and phylogenic analysis. Loss on drying is 7.39 ± 1.26, and qualitative results show the presence of alkaloids, flavonoids, and tannins, and HPTLC profile shows 6, 6, and 5 spots at short UV, long UV, and visible light, respectively. Conclusion: Leea macrophylla is a shrub with unipinnate compound leaves, and the leaflets possess 5-7 nerves arising from petiolule. Microscopic typical features of leaves belonging to genus Leea are the presence of raphides sacs, secretory cells, acicular crystals, and warty trichomes. The results obtained from the phytochemical study will be helpful in the determination of strength of purity and also for further standardization of the plant.

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  1. Retnam KR, Martin P. Ethnomedicinal Plants. Jodhpur: Agrobios, 2008; p.1.
  2. Brahmam M, Saxena HO. Flora of Orissa. Orissa: Regional Research Laboratory, 1994; vol. I, p.340.
  3. Abu NN, Atiar Md R, Nazim UA, et al. Whole Leea macrophylla ethanolic extract normalizes kidney desposits and recovers renal impairments in an ethylene glycol—induced urolithiasis model of rats. Asian Pac J Trop Med 2012;5(7);533-538, doi: 10.1016/S1995-7645(12)60094-7.
  4. Nath V, Khatri PK. Traditional knowledge on ethno-medicinal uses prevailing in tribal pockets of Chhindwara and Betul Districts, Madhya Pradesh, India. Afr J Pharm Pharmacol 2010;4(9):662-670.
  5. Khare CP. Indian Medicinal plants (an illustrated dictionary). Berlin (Germany): Springer Publishers; 2007. p.367.
  6. Haq R, Shamsi S. Report of association of mycoflora with leaves and stems of Leea macrophylla Roxb.ex harnem from Bangladesh. J Bangladesh Acad Sci 2012;36(2):257-262.
  7. Farug AA, Ibrahim M, Ayesha M, et al. Pharmacological and phytochemical screenings of ethanol extract of Leea macrophylla Roxb. Innovations in Pharmaceuticals and Pharmacotherapy 2014;2(1):321-327.
  8. Jadhao KD, Wadekar MP, Mahalkar MS. Comparative study of availability of vitamins from Leea macrophylla Roxb. Biosci Biotechnol Res Asia 2009;6:847-849.
  9. Trease and Evans WC. Pharmacognosy. 16th ed. London: Saunders Elsevier; 2009. pp.567-568.
  10. Anonymous. The Ayurvedic Pharmacopoeia of India. Part-II, Vol-II. 1st ed. New Delhi: Government of India, Ministry of Health and Family Welfare, Department of AYUSH; 2008.
  11. Khandelwal KR. Practical Pharmacognosy. 19th ed. Pune: Nirali Prakashan; 2008.
  12. Egon S. Thin Layer Chromatography. Springer Publication, 1969, reprint 2005.
  13. Sarvade DD, Acharya RN. An appraisal on Hastikarnapalasha vis-à-vis Leea macrophylla Roxb. ex Hornem. W.s.r. to ayurveda literature and different flora‘s of medicinal plants. International journal of Ayurveda and Pharmaceutical Chemistry 2019;11(1):16-30.
  14. Metcalfe CR, Chalk L. Anatomy of the dicotyledons. Vol-I, Great Britain: Oxford University press; 1950. p.414-419.
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