Journal of Drug Research in Ayurvedic Sciences

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VOLUME 3 , ISSUE 2 ( June, 2018 ) > List of Articles


Comparative Evaluation of Thin-layer Chromatography/ High-performance Thin-layer Chromatography Fingerprint Profiles of Successive Extracts in Development of Standardization Protocol for Ayurvedic, Siddha or Unani Formulations

Maheshwari Balasundaram, Ajay K Meena, Ravindra Singh, Raju Ilavarasan

Keywords : High-performance thin-layer chromatography (HPTLC) fingerprint, Phytochemicals, Polyherbal formulation, Standardization, Successive extracts, Trikatu churna

Citation Information : Balasundaram M, Meena AK, Singh R, Ilavarasan R. Comparative Evaluation of Thin-layer Chromatography/ High-performance Thin-layer Chromatography Fingerprint Profiles of Successive Extracts in Development of Standardization Protocol for Ayurvedic, Siddha or Unani Formulations. J Drug Res Ayurvedic Sci 2018; 3 (2):77-84.

DOI: 10.5005/jp-journals-10059-0039

License: CC BY-NC-SA 3.0

Published Online: 00-06-2018

Copyright Statement:  Copyright © 2018 Jaypee Brothers Medical Publishers (P) Ltd.


Aim: Trikatu churna is an important and unique polyherbal formulation used for various therapeutic indications. The World Health Organization (WHO) has emphasized the need to ensure the quality of medicinal plant products and herbal formulations by using any modern controlled techniques. High-performance thin-layer chromatography (HPTLC) is a frequently used technique to develop the chromatographic fingerprints. Therefore, the present study was carried out for quality evaluation of Trikatu churna through the development of standardization protocol with comparative HPTLC fingerprint profiles of successive extracts besides routine analysis. Materials and methods: Successive extracts of various solvents of polarity in increasing order through soxhlet apparatus for Trikatu churna and its authenticated raw materials were prepared; comparative chromatographic fingerprint profiles developed along with the evaluation of qualitative and quantitative tests parameters have been carried out by using the Ultraviolet (UV)–visible spectrophotometer, flame photometer, and atomic absorption spectrophotometer. Results: The following test parameters physicochemical parameters, evaluation of qualitative, quantitative phytochemicals such as total tannins, sugars and phenols, micronutrients such as sodium (Na), potassium (K); safety parameters such as microbial load, heavy metals and aflatoxins; comparative TLC and HPTLC fingerprint profiles of successive extracts of ingredients in increasing the polarity order of the solvents (n–hexane to alcohol) with respect to that of consecutive extracts of the final polyherbal formulation have been carried out for the quality evaluation. Conclusion: Trikatu churna was evaluated for its identity and purity by the systematic analysis of the above parameters for raw botanical ingredients and formulation in the course of development of standardization protocol for quality control.

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